What is the mood and theme of Annunciation by Leonardo da Vinci?

The mood is just how you think the painting makes you feel. The theme is just what the title says, The Annunciation. An Annunciation is an image of the moment where the angel comes to tell Mary that she is pregnant with the Christ child. Since the theme explains what is going on the painting, that would be the theme. And now since I've described what the title of the painting means you can see how that relates to the image.